▶  Ngokusebenzisa isethi yesitayela sethu, izitayela zochromatography eziningi zingafakwa, ekuncintiseni imali. Isikhala esithathu kancane kancane kwezinyawo zokuxhumana okwesibili kanye nezinyawo zezinkinobho, futhi isikhala kuyinto kuphela ngepaki yoku-2/3; Kwenza okuthuthukile izidingo zezindawo zokufaka.
▶  Isikhala sokufaka isikhathi sifuphi, kungenzeka okungenasidingo sokuthatha okwesibili nokufaka okwesibili. Iyathatha isicelo esiphakathi, futhi zonke izinto zingatholakala ngokubuka ngokwezulu ngenxa yezinkinga zezindlela zokusebenza, kwenza kube lula ukulawula ngokwesikhathi. Ukuya ekubuyisweni kwesitayela sakho ngenxa yokufaka nokufaka kwesitayela sokuchromatography sokuthuthukisa, okungathinta ukuthuthukisa kwesitayela;
Isihlangu sesiphiston sifuna ekuboniseni nesitayela sokufaka futhi sikhokhela isikhala sokufaka, ekuphatheni isidingo sokuthuthukisa esikhathini eside ngesitayela sokuchromatography sokuthuthukisa okwesibili futhi ekwenzeni kube lula ukuthuthukisa kwalo; Isistimu se-hydraulic isekelwe ekunqunyweni kwesistimu yonke futhi isetshenziswa isicelo esiphakathi kahle ukuze kwenzeke okuningi nokungathinti;
▶  Isistimu sokwenza nokuthatha okwenziwe ngokuthuthukiswa okwenziwe kahle kungenzela ukuthuthukisa okuphelele kanye nekusiza kwesitayela sokuchromatography. Lolu hlobo lwemvelo lwenziwe ngokuthuthukiswa okwenziwe ngokuthuthukiswa, futhi inombolo yezithombe yezithombe yezithombe yezithombe (10um C18 packing) kuyafika kwi-40000/m, nesimethri esiphakathi kancane kunayo 1.3.